Course program, A.Y. 2014-15
Lesson | Date | Topics | Linked slides and useful links |
1 | February 23rd, 2015 | Overview of cell structure.
Cell-cycle, and cell proliferation. DNA basics. Classroom exercises: measurement of the doubling time of leukemic cells. Classroom demonstration: extraction of DNA from cells. |
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2 | March 2nd, 2015 | DNA damage and mutations. Basics concepts of radiation damage
in cells, and in particular:
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3 | March 9th, 2015 | Effects of radiation. Stochastic and non-stochastic effects.
Linear quadratic model. Lea's target model. Early and late
effects of exposure to radiation. Linear-quadratic model for
early- and late-responding tissues. Mortality rate and lethal
dose LD50/30. Cell survival curves and dose-response curves.
Endpoints. Dose-response curves for low-LET radiation and
high-LET radiation. RBE (Relative Biological Effectiveness). |
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4 | March 16th, 2015 | Survival curves for different cell types and different LET.
Dependence of RBE on LET. Threshold effects. Dependence of
survival curves on phase. Oxygen effect. The problematic nature
of low-level radiation. Dependence of LD50 on dose-rate.
Epidemiological evidence of cancer induction from exposure to
low-level radiation. Tumor Control Probability. Normal Tissue
Complication Probability. Influence of dose-rate. Fractionation.
Biologically Effective Dose. Isoeffect formulas and EQD(2Gy).
Radiosensitizers. QUANTEC guidelines. |